Isolation of serum albumin-synthesizing polysomes from rat liver |
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| Authors: | M Takagi K Ogata |
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| Institution: | 1. Laboratory of Radiation Genetics, Faculty of Agriculture University of Tokyo, Tokyo, Japan;2. Department of Biochemistry, Niigata University School of Medicine Niigata, Japan |
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| Abstract: | The procedures for the purification of rat liver polysomes synthesizing serum albumin was developed, employing the quantitative precipitin method with rat serum albumin as a carrier and its antibody, and ribonuclease inhibitor from rat liver. The addition of ribonuclease inhibitor to polysomes during the incubation with antibody was found to prevent their degradation. Under these conditions, about 12 % of the membrane-bound polysomes of rat liver was found in the specific precipitate of serum albumin and its antibody, while a negligible amount of free polysomes was precipitated. It is concluded that polysomes synthesizing serum albumin are isolated by this method. |
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