High-level production of heterologous protein by engineered yeasts grown in cottage cheese whey. |
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| Authors: | C Maullu G Lampis A Desogus A Ingianni G M Rossolini R Pompei |
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| Institution: | Dipartimento di Scienze Mediche, Cattedra di Microbiologia Applicata, Università di Cagliari, 09124 Cagliari, Italy. |
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| Abstract: | Cottage cheese whey is a cheese industry by-product still rich in proteins and lactose. Its recycling is seldom cost-effective. In this work we show that the lactose-utilizing yeast Kluyveromyces lactis, engineered for production of recombinant human lysozyme, can be grown in cottage cheese whey, resulting in high-level production of the heterologous protein (125 microg/ml). |
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