Genetic and proteomic analyses of CO utilization by <Emphasis Type="Italic">Methanosarcina acetivorans</Emphasis> |
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Authors: | Michael Rother Ellen Oelgeschläger William W Metcalf |
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Institution: | 1.Institut für Molekulare Biowissenschaften, Abteilung Molekulare Mikrobiologie und Bioenergetik,Johann Wolfgang Goethe-Universit?t Frankfurt,Frankfurt am Main,Germany;2.Department of Microbiology,University of Illinois at Urbana-Champaign,Urbana,USA |
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Abstract: | Methanosarcina
acetivorans, a member of the methanogenic archaea, can grow with carbon monoxide (CO) as the sole energy source and generates, unlike
other methanogens, substantial amounts of acetate and formate in addition to methane. Phenotypic analyses of mutant strains
lacking the cooS1F operon and the cooS2 gene suggest that the monofunctional carbon monoxide dehydrogenase (CODH) system contributes to, but is not required for,
carboxidotrophic growth of M. acetivorans. Further, qualitative proteomic analyses confirm a recent report (Lessner et al., Proc Natl Acad Sci USA, 103:17921–17926,
2006) in showing that the bifunctional CODH/acetyl-CoA synthase (ACS) system, two enzymes involved in CO2-reduction, and a peculiar protein homologous to both corrinoid proteins and methyltransferases are synthesized at elevated
levels in response to CO; however, the finding that the latter protein is also abundant when trimethylamine serves as growth
substrate questions its proposed involvement in the reduction of methyl-groups to methane. Potential catabolic mechanisms
and metabolic adaptations employed by M. acetivorans to effectively utilize CO are discussed. |
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Keywords: | Methanosarcina acetivorans Carbon monoxide Carbon monoxide dehydrogenase |
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