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Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion
Authors:A Mann  R P Yadav  J Singh  D Kumar  B Singh  P S Yadav
Institution:1. Buffalo Physiology and Reproduction Division, Central Institute for Research on Buffaloes, Hisar, 125001, Haryana, India
2. Indian Veterinary Research Institute, Regional Station, Palampur, 176061, India
Abstract:Stem cells present an important tool in livestock assisted reproduction and veterinary therapeutic field such as tissue engineering. We report for the first time isolation of pluripotent stem cell-like cells expressing pluripotency markers (alkaline phospahatase, OCT-4, NANOG and SOX-2) from the amnion of water buffalo (Bubalus bubalis). The cells showed no apparent abnormalities in their chromosomal profiles before and after cryopreservation. The cytochemical staining revealed that pluripotent cells were capable of undergoing directed differentiation in vitro into osteocytes. It could be inferred that amnion-derived pluripotent stem cell-like cells can be isolated, cultured for many passages and differentiated into mesoderm lineage, and may be an alternative source to mesenchymal stem cells. These cells can have applications in assisted reproduction, developmental biological and regenerative medicine.
Keywords:Amnion cells  Buffalo  Differentiation  Pluripotency  Stem cells
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