Identification of insulin variants using Raman spectroscopy |
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Authors: | Ortiz Corasi Zhang Dongmao Xie Yong Davisson V Jo Ben-Amotz Dor |
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Affiliation: | Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA. |
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Abstract: | Drop coating deposition Raman (DCDR) spectroscopy is used to obtain high-quality normal Raman spectra from small volumes (10 microl) of dilute insulin solutions (3-400 microM) for spectral identification and chromatographic detection. The results are used to demonstrate the spectroscopic classification (identification) of three natural insulin variants-human, bovine, and porcine-that differ by between one and three amino acid residues. DCDR measurements were performed on solutions obtained from reverse phase high-performance liquid chromatography (RP-HPLC) eluent fractions, either before or after lyophilization. Classification is demonstrated using replicate DCDR measurements, followed by normalized Savitsky-Golay second derivative preprocessing and partial least squares training with either leave-one-out or batch-to-batch testing. |
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Keywords: | Insulin Variants Raman Classification |
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