| Vitek—AMS系统检测超广谱β—内酰胺酶的评价 |
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| 引用本文： | 陈茶,黄彬. Vitek—AMS系统检测超广谱β—内酰胺酶的评价[J]. 中国微生态学杂志, 2001, 13(4): 231-232 |
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| 作者姓名： | 陈茶 黄彬 |
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| 作者单位： | 1. 广东省中医院检验科
2. 中山医科大学附属第一医院医学检验部 |
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| 摘 要： | 目的：评价Vitek-AMS系统检测超广谱β-内酰胺酶（ESBLs)方法的敏感性和特性。方法：用Vitek－32仪器法、双纸片协同试验法检测45株ESBLs阳性和45株ESBLs阴性菌株，并将结果进行比较。结果：Vitek－32仪器法检出45株ESBLs阳性株中的43株，而双纸片协同试验法仅检出38株，45株ESBLs阴性株用两法检测均为阴性。Vitek－AMS仪器法检测ESBLs的方法敏感性为95．5％，特异性为100％，而双纸片协同试验法分别为84．4％和100％。结论：Vitek－AMS系统检测ESBLs的方法敏感性和特异性均优于双纸片同试验法，且操作简便，有条件的实验室可将其作为初筛ESBLs的首选方法。
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| 关 键 词： | β－内酰胺酶类 抗药性 微生物 Vitek－AMS系统 检测 |
| 文章编号： | 1005-376(2001)04-0231-02 |
| 修稿时间： | 2001-02-26 |
EVALUATION OF THE SENSITIVITY AND SPECIFICITY FOR THE DETECTION OF EXTENDED SPE CTRUM BETA-LACTAMASES BY VITEK-AMS SYSTEM |
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| CHEN Cha,HUANG Bin. EVALUATION OF THE SENSITIVITY AND SPECIFICITY FOR THE DETECTION OF EXTENDED SPE CTRUM BETA-LACTAMASES BY VITEK-AMS SYSTEM[J]. Chinese Journal of Microecology, 2001, 13(4): 231-232 |
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| Authors: | CHEN Cha HUANG Bin |
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| Abstract: | Objective:To evaluate the sensitivity and specificity for the detection of extended spectrum beta lactamases(ESBLs)by Vitek AMS system Methods:45 ESBLs producing strains and 45 non ESBLs producing strains were detected by Vitek 32 system and the double disk synergy test Results:43 of 45 ESBLs producing strains were found to be ESBLs positive by Vitek 32 system, however, only 38 ESBLs producing strains were detected positive by the double disk synergy test 45 non ESBLs producing strains were found to be ESBLs negative by two methods The sensitivity?specificity for detection of ESBLs by Vitek 32 system and the double disk synergy test were 95 5%?100%?84 4%?100%,respectively Conclusions:The sensitivity and specificity for the detection of ESBLs by Vitek 32 system were higher than the double disk synergy test Vitek AMS system is suitable for screening ESBLs producing strains routinely in hospitals |
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| Keywords: | Beta lactamases Drug resistamce microbial |
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