Functional human Th17 clones with WT1-specific helper activity |
| |
Authors: | Sho Tachino Fumihiro Fujiki Yoshihiro Oka Akihiro Tsuboi Soyoko Morimoto Yu-Hung Lin Taichi Tamanaka Kenta Kondo Hiroko Nakajima Sumiyuki Nishida Naoki Hosen Yusuke Oji Atsushi Kumanogoh Haruo Sugiyama |
| |
Institution: | 1. Department of Respiratory Medicine, Allergy and Rheumatic Diseases, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, Japan 2. Department of Cancer Immunology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, Japan 3. Department of Immunopathology, WPI Immunology Frontier Research Center, Osaka University, 3-1 Yamadaoka, Suita, Osaka, Japan 4. Department of Cancer Immunotherapy, Osaka University Graduate School of Medicine, 3-1 Yamadaoka, Suita, Osaka, Japan 5. Department of Functional Diagnostic Science, Osaka University Graduate School of Medicine, 1-7 Yamadaoka, Suita, Osaka, Japan 6. Department of Cancer Stem Cell Biology, Osaka University Graduate School of Medicine, 1-7 Yamadaoka, Suita, Osaka, Japan
|
| |
Abstract: | Th17 plays important roles in the pathogenesis of various inflammatory and autoimmune diseases. Although the importance of Th17 in tumor immunity has also been suggested, precise roles of tumor-associated antigen-specific Th17 still remain poorly understood, especially in humans. We previously identified WT1332, a 16-mer helper epitope derived from tumor-associated antigen Wilms’ tumor gene 1 (WT1) product, and WT1332-specific Th1 clones were established. In the present study, WT1-specific Th17 clones were established by the stimulation of peripheral blood mononuclear cells with the WT1332 helper peptide under human Th17-polarizing conditions. The WT1-specific Th17 clone exhibited the helper function for proliferation of conventional CD4+ T cells in the antigenic stimulation-specific manner. This is the first report of establishment of functional Th17 clones with both antigen (WT1332) specificity and antigen-specific helper activity. Th17 clones established here and the method to establish antigen-specific Th17 clones should be a useful tool to further analyze the roles of human Th17 in tumor immunity. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|