Proenkephalin A 119-159, a stable proenkephalin A precursor fragment identified in human circulation |
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Authors: | Ernst A Köhrle J Bergmann A |
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Institution: | SphingoTec GmbH, Tulpenweg 6, D-16556 Borgsdorf, Germany. a.ernst@sphingotec.de |
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Abstract: | In this report, we describe a newly developed sandwich immunoassay using antibodies against the proenkephalin A 119-159 peptide (PENK A 119-159). PENK A 119-159 immunoreactivity was detectable in the circulation of human blood donors and in cerebrospinal fluid (CSF) of patients without a neurologic disorder. The concentration was about 100 times higher in CSF than in serum. Analytical reversed phase HPLC revealed that PENK A 119-159 is the main immunoreactivity in human circulation and CSF. Moreover, PENK A 119-159 is stable in vitro for at least 48 h at room temperature as compared to the low stability of the peptides methionine- and leucine-enkephalin. This suggests the use of PENK A 119-159 measurement as surrogate molecule for the release of the mature peptides derived from proenkephalin A. |
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