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Quantitative determination of the juvenile hormones in the haemolymph of Locusta migratoria during normal development and after implantation of corpora allata
Authors:L Huibregtse-Minderhoud  MAM Van den Hondel-Franken  AC Van der Kerk-Van Hoof  HWA Biessels  CA Salemink  DJ Van der Horst  AMTh Beenakkers
Institution:1. Laboratory of Organic Chemistry, State University of Utrecht, PO Box 5055, 3502 JB Utrecht, The Netherlands;2. Laboratory of Chemical Animal Physiology, State University of Utrecht, 8 Padualaan, 3508 TB Utrecht, The Netherlands
Abstract:Simultaneous quantitative determination of the three naturally occurring juvenile hormones in insects (JH-I, JH-II and JH-III) was performed on haemolymph samples of both normally developing locusts and locusts implanted with active corpora allata, using capillary gas chromatography with electron capture detection.In fourth instar female larvae, 24–48 hr after the third ecdysis, as well as in adult females, 18 days after the imaginal ecdysis, only JH-III was detected. In fifth instar female larvae JH-III was present in very low concentrations, if at all.After implantation of four pairs of corpora allata taken from young fourth instar female larvae or one pair or corpora allata taken from adult females into fifth instar female larvae 0–24 hr after ecdysis, an elevation of the JH-III titre was observed. Neither JH-I nor JH-II could be detected. The amount of JH-III, already elevated 2 hr after implantation, remained high for several days in comparison to that of control insects. On the third day after the subsequent moult the JH-III level was comparable to that of normally developing fifth instar larvae. Factors involved in the achievement of the haemolymph JH-titre are discussed.
Keywords:juvenile hormones  capillary gas chromatography  electron capture detection  corpora allata  flight muscles
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