Purification and properties of the Bombyx mori nuclear polyhedrosis virus liberated from polyhedra by dissolution with silkworm gut juice |
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Authors: | Takeshi Kawarabata Masako Funakoshi Yoshinobu Aratake |
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Affiliation: | Institute of Biological Control, Faculty of Agriculture, Kyushu University, Higashi-ku, Fukuoka 812, Japan;Sericulture Experiment Station, Kyushu Branch, Uekimachi, Kamoto-gun, Kumamoto Prefecture 861-01, Japan |
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Abstract: | Occluded virions of the Bombyx mori nuclear polyhedrosis virus were efficiently liberated from polyhedra by dissolution with the silkworm gut juice. The liberated virions were purified by sucrose density gradient centrifugation and the bands of enveloped virions were observed in the gradients. There was no functional difference between the gut juice-liberated and the carbonate-liberated virions. Disruption of enveloped virions by the gut juice was observed, but the formation of nucleocapsids from the degradation of the occluded virions was not detected. High yields of the enveloped virions from the polyhedra dissolved by the gut juice was obtained by separating the virions through sucrose density gradient centrifugation immediately after the dissolution of the polyhedra. Many factors, e.g., rearing seasons, silkworm strains, and rearing conditions, affect the polyhedra-dissolving property of the larval gut juice. |
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Keywords: | Bombyx mori nuclear polyhedrosis virus silkworm gut juice polyhedra-dissolving activity gut juice-liberated virus peroral hemocoelic infectivities sucrose density gradient centrifugation |
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