Comparative analysis of the alkali-liberated components of the Hyphantria cunea and the Diacrisia virginica granulosis viruses |
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Authors: | D.G. Boucias G.L. Nordin |
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Affiliation: | Department of Entomology, University of Kentucky, Lexington, Kentucky 40546 USA |
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Abstract: | The biochemical and biophysical characteristics of the closely related Diacrisia virginica and Hyphantria cunea granulosis virus isolates were examined. Sucrose gradient sedimentation patterns of alkali-solubilized DGV and HcGV capsules were identical. The top, middle, and bottom fractions from either viral isolate were infectious when injected into susceptible host larvae. Electrophoretic analysis of alkaline-solubilized granulin extracts demonstrated that both viruses contain alkaline proteolytic activity. The major granulin protein (~28,000 daltons) of both isolates comigrated in a SDS-PAGE. Electrophoretic separation of the virus proteins demonstrated some quantitative differences between the two granulosis viruses. The enveloped nucleocapsids and the nucleocapsids of the two viruses were morphologically indistinguishable. |
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Keywords: | Granulosis virus granulin insect virus |
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