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Effect of repeated subculturing on agar and passaging through an insect host on pathogenicity,morphology, and growth rate of Verticillium lecanii
Authors:R.A. Hall
Affiliation:Glasshouse Crops Research Institute, Worthing Road, Littlehampton, Sussex, England
Abstract:Repeated subculturing on different agars of single- and multispore isolates of a strain of Verticillium lecanii resulted in physiological and gross morphological changes but no significant attenuation of virulence. Furthermore, passaging through an aphid host, Macrosiphoniella sanborni, did not enhance virulence. Precautions are suggested to avoid any changes in characteristics of fungal strains in axenic culture.
Keywords:fungus virulence of
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