鲇肠道黏液细胞的类型、分布、发育及分泌方式研究

利用阿利新蓝-过碘酸雪夫氏试剂(AB-PAS,AB染液pH2．6),对鲇(Silurus asotus)肠道黏液细胞的类型与分布进行了研究,同时采用扫描和透射电镜,研究了黏液细胞的发育与分泌方式。结果显示:鲇肠道黏液细胞染色后呈深蓝色、淡蓝色和浅蓝色,主要含有酸性黏多糖:形态上进一步可将其分为囊状、梨状和杯状三种形态。肠道中黏液细胞的分布密度以中肠最高。其次是后肠,前肠最低;肠道黏液细胞由肠黏膜基底层细胞衍生后,不断向肠腔表层推移而发育成熟;发育过程中黏液细胞内富含大小不等的黏原颗粒,其分泌方式为顶浆分泌。

STUDY ON THE TYPE, DISTRIBUTION, DEVELOPMENT AND SECRETING ACTIVITY OF MUCOUS CELLS IN THE INTESTINE TRACT OF CATFISH (SILURUS ASOTUS)

The type and distribution of mucous cells stained with AB & PAS (pHAB=2.6) in the intestine tract of silurus asotus (body length 16.4-21.0cm, body weight 33.6-64.3g) have been studied. The development and secreting activity of mucous cells have been investigated using scanning and transmission electron microscopy. The results showed that the mucous cells were dark blue,weak blue and cambridge blue in colour after being stained with AB & PAS(pHAB=2.6); it mainly contained acid mucopolysaccharides; the mucous cells had 3 shapes i.e. capsule-shaped, pear-shaped and cup-shaped. The capsule-shaped mucous cells were distributed in the bottom of the epithelia of the intestine, and its diameter was about 4.40 microm. The pear-shaped mucous cells were distributed in the middle-layer of the epithelia of the intestine, and its vertical range and sidelong was about 6.00 microm and 3.40 microm respectively. The cup-shaped mucous cells were distributed in the top of the epithelia of the intestine, and its vertical range and sidelong was about 12.00 microm and 4.60 microm respectively. The density of mucous cells presented an increasing trend from the anterior(low) to posterior(high) and middle(higher) segment. After derived from bottom cells, the mucous cells evolved gradually from fundus film to exterior of mucous membrane. The mucous cells in developing were filled with plenty of big and small mucosity particulates, and the secreting method of mucous cells was peak serosity secretion.