5S ribosomal RNA is contained within a 25 S ribosomal RNA that accumulates in mutants of Escherichia coli defective in processing of ribosomal RNA. |
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Authors: | B K Ghora D Apirion |
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Institution: | Department of Microbiology and Immunology Division of Biology and Biomedical Sciences Washington University School of Medicine St. Louis, Mo. 63110, U.S.A. |
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Abstract: | A temperature-sensitive mutant strain of Escherichia coli defective in two RNA processing enzymes, RNase III and RNase E (rnc. rne), fails to produce normal levels of 23 S and 5 S rRNA at the non-permissive temperature. Instead, a molecule larger than 23 S is produced. This molecule, designated 25 S rRNA, can be processed in vitro to produce p5 rRNA. These findings further our understanding of the overall processing events of ribosomal RNA which take place in the bacterial cell. |
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