| 大熊猫乳酸脱氢酶同工酶H_4的分离纯化和某些性质的研究 |
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| 引用本文： | 彭浪,张龙翔. 大熊猫乳酸脱氢酶同工酶H_4的分离纯化和某些性质的研究[J]. 中国生物化学与分子生物学报, 1995, 11(1): 85-90 |
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| 作者姓名： | 彭浪 张龙翔 |
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| 作者单位： | 北京大学生命科学学院 |
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| 摘 要： | 采用８－（６－氨己基）－氨基－５＇－ＡＭＰＳｅｐｈａｒｏｓｅ亲和层析法和ＤＥＡＥ－Ｓｅｐｈａｒｏｓｅ离子交换层析法从大熊猫心肌中分离纯化出了乳酸脱氢酶同工酶Ｈ４．纯化的大熊猫ＬＤＨ－Ｈ４，比活为４４５Ｕ／ｍｇ蛋白，经ＳＤＳ－ＰＡＧＥ，ＰＡＧＥ，等电聚焦电泳鉴定均为一条带，其亚基分子量为３６０００，等电点为５．４５．经测定大熊猫ＬＤＨ－Ｈ亚基Ｎ端被封闭，Ｃ端氨基酸残基经测定为Ｌｅｕ．氨基酸组成分析表明每个亚基含有５个Ｃｙｓ，９个Ｍｅｔ．
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| 关 键 词： | 大熊猫；乳酸脱氢酶；同工酶 |
| 收稿时间： | 1995-02-20 |
Separation and Some Characteristics of LactateDehydrogenase Isozyme H_4 of Giant Panda |
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| Peng,Lang,Zhang,Long-Xiang. Separation and Some Characteristics of LactateDehydrogenase Isozyme H_4 of Giant Panda[J]. Chinese Journal of Biochemistry and Molecular Biology, 1995, 11(1): 85-90 |
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| Authors: | Peng Lang Zhang Long-Xiang |
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| Affiliation: | (School of Life Science, Peking Universaity, Beijing 100871 |
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| Abstract: | Lactate dehydrogenase (LDH EC 1.1.1.27) isozyme H4 was separated and purified from giant panda heart muscle by 8-(6-aminohexyl)-amino-5’-AMPSepharose 4B affinity chromatography and DEAE-Sepharose ion exchange chromatography. 34mg LDH-H4 were obtained from 100g heart muscle. Its specific activity was 445U/mg. The purified LDH-H4 was shown to be homogenous by SDS-PAGE, PAGE and IEF with a pI of 5.45. The subunit has a molecular weight of 36kD. There are 5 Cys and 9 Met in each subunit according to its amino acid compositon analysis.It has a blocked N-terminal and its C-terminal residue is Leu. |
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| Keywords: | Lactate dehydrogenase Isozyme Giant panda |
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