| 内生青霉菌对黄花蒿组培苗生长和青蒿素合成的影响 |
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| 引用本文： | 袁亚菲,董婷,王剑文.内生青霉菌对黄花蒿组培苗生长和青蒿素合成的影响[J].氨基酸和生物资源,2011,33(4):1-4. |
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| 作者姓名： | 袁亚菲 董婷 王剑文 |
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| 作者单位： | 苏州大学药学院,江苏苏州,215123;苏州大学药学院,江苏苏州,215123;苏州大学药学院,江苏苏州,215123 |
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| 基金项目： | 国家教育部留学基金项目(K513201011); 苏州大学东吴学者计划(14317363); 江苏省六大人才高峰项目(Bu132802) |
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| 摘 要： | 从黄花蒿茎中分离得到了17株内生真菌,其中内生青霉菌(Penicilliumsp.Y2)能有效促进黄花蒿组培苗生长及青蒿素合成。内生青霉菌悬浮培养5d后,分别将培养液与菌丝匀浆后经过高压灭菌处理,或将培养液经过高压灭菌、过滤除菌处理获得3种内生菌诱导子(A、B和C)。结果表明,3种内生菌诱导子对植株生长、抗氧化酶活性及青蒿素合成都有促进作用,诱导子C青蒿素合成诱导效果最好,可促进黄花蒿组培苗的干重增长44.44%、可溶性糖含量提高38.24%,诱导超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性,从而提高青蒿素合成达58.86%,黄花蒿组培苗青蒿素含量达4.701mg.g-1(干重)。
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| 关 键 词： | 黄花蒿 青蒿素 内生真菌 诱导子 |
Effect of Endophytic Penicillium sp. Y2 on Growth and Artemisinin Biosynthesis of Plantlets in Tissue Cultures of Artemisia annua L. |
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| YUAN Ya-fei,DONG Ting,WANG Jian-wen.Effect of Endophytic Penicillium sp. Y2 on Growth and Artemisinin Biosynthesis of Plantlets in Tissue Cultures of Artemisia annua L.[J].Amino Acids & Biotic Resources,2011,33(4):1-4. |
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| Authors: | YUAN Ya-fei DONG Ting WANG Jian-wen |
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| Institution: | YUAN Ya-fei,DONG Ting,WANG Jian-wen(College of pharmaceutical Sciences,Soochow University,Suzhou 215123,China) |
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| Abstract: | 17 isolates of endophytic fungi were isolated from the stems of Artemisia annua L.Penicillium sp.Y2 could effectively promote the growth and artemisinin accumulation in the plantlets in tissue cultures of A.annua.After 5 days of broth culture,the fungal mycelium and culture medium were prepared for elicitors,including elicitor A(autoclaved conditioned homogenates of mycelia filtrates and medium),elicitor B(autoclaved conditioned medium) and elicitor C(filtrated conditioned medium).The results showed that al... |
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| Keywords: | Artemisia annua L artemisinin endophytic fungi elicitor |
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