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| 李砧木Marianna离体叶片再生体系优化研究 | |
| 引用本文： | 周厚成,赵霞,李明,郭蔼光.李砧木Marianna离体叶片再生体系优化研究[J].西北植物学报,2011,31(10):2113-2118. |
| 作者姓名： | 周厚成 赵霞 李明 郭蔼光 |
| 作者单位： | 1. 西北农林科技大学生命科学学院,陕西省农业分子生物学重点实验室,陕西杨陵712100;中国农业科学院郑州果树研究所,郑州450009 2. 中国农业科学院郑州果树研究所,郑州,450009 3. 西北农林科技大学生命科学学院,陕西省农业分子生物学重点实验室,陕西杨陵712100 |
| 基金项目： | 国家自然科学基金(30571282) |
| 摘 要： | 以李砧木‘Marianna’试管苗新梢顶端第1片叶为外植体,研究激素组合、基本培养基种类及外植体类型等对不定芽再生的影响。结果表明:1/2 MS基本培养基和WPM培养基再生率显著高于MS和SH培养基;叶片附带叶柄的外植体再生率和再生不定芽数显著高于叶柄和切除叶柄的叶片外植体;最佳再生培养基为1/2MS+2.0mg/L TDZ+0.1 mg/L IBA+0.25%琼脂+3.0%蔗糖,最高再生率和再生不定芽数分别为81.7%和7.46±1.38个;最佳生根培养基为1/2MS+0.5~1.0 mg/L IBA,能获得96.7%生根率、较高的生根数和根长。 |
| 关 键 词： | 李砧木 不定芽再生 生根 |
Optimizing System of Plant Regeneration from in vitro Leaves of Plum Rootstock ‘Marianna' ( Prunus cerasi f era × P.munsoniana ) |
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| ZHOU Hou-cheng,ZHAO Xia,LI Ming,GUO Ai-guang.Optimizing System of Plant Regeneration from in vitro Leaves of Plum Rootstock ‘Marianna' ( Prunus cerasi f era × P.munsoniana )[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(10):2113-2118. | |
| Authors: | ZHOU Hou-cheng ZHAO Xia LI Ming GUO Ai-guang |
| Institution: | ZHOU Hou-cheng1,2,ZHAO Xia2,LI Ming2,GUO Ai-guang1(1 College of Life Sciences,Northwest A&F University,Key Laboratory of Plant Molecular Biology of Shaanxi Province,Yangling,Shaanxi 712100,China,2 Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China) |
| Abstract: | A complete protocol for adventitious shoot regeneration in vitro was developed from the leaves of plum rootstock ’Marianna’(Prunus cerasifera×P.munsoniana).The first leaves along with their petioles from in vitro-grown shoots were used as explants.The regeneration percentage was influenced by plant growth regulators,basal medium and explant type.The result showed that the regeneration percentages were significantly higher on 1/2MS or WPM medium than on MS medium or SH medium.Explants of leaves with petiole were more effective than leaves without petiole or petiole explants in terms of regeneration.The highest regeneration percentage at 81.7% and a mean of 7.46±1.38 shoots were obtained on 1/2 MS medium supplemented with 2.0 mg/L thidiazuron,0.1 mg/L IBA,0.25% agar and 3% sugar.Adventitious shoots were rooted and the better rooting percentage at 96.7% were obtained on 1/2MS medium supplemented with 0.5～1.0 mg/L IBA.The rooted plantlets survived after acclimatization in the greenhouse. |
| Keywords: | plum rootstock adventitious shoots rooting |
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