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| Notch信号通路调控猕猴胚胎干细胞的胆管样细胞分化(英文) | |
| 引用本文： | Jin LF,Ji SH,Yang JF,Ji WZ. Notch信号通路调控猕猴胚胎干细胞的胆管样细胞分化(英文)[J]. 动物学研究, 2011, 32(4): 391-395. DOI: 10.3724/SP.J.1141.2011.04391 |
| 作者姓名： | Jin LF Ji SH Yang JF Ji WZ |
| 作者单位： | 1. 绍兴文理学院生命科学学院,浙江绍兴312000;中国科学院昆明动物研究所云南省动物生殖生物学重点实验室,云南昆明650223 2. 中国科学院昆明动物研究所云南省动物生殖生物学重点实验室,云南昆明,650223 3. 中国科学院昆明动物研究所云南省动物生殖生物学重点实验室,云南昆明650223;温州医学院生命科学学院,浙江温州325035 |
| 基金项目： | supported by research grants from Zhejiang Natural Sciences Foundation of China (Y2110911; Y2080996); the National Key Technologies R&D Program of China (2007CB947701) |
| 摘 要： | 猕猴胚胎干细胞(rhesus monkey embryonic stem(rES))与人胚胎干细胞有相似的生物学特性,因此是理想的临床前研究的替代模型。Notch信号通路在胆管及胆管上皮细胞的形成中有重要的作用,然而,有关Notch信号通路在ES细胞的胆向分化中的作用了解甚少。该实验以rES为模型,对Notch信号通路对ES细胞的胆向分化过程中的作用进行了较为系统的研究。rES在细胞因子ActivinA诱导作用下产生约80%的限定性内胚层细胞。以Matrigel作为细胞外基质,在含BMP4和FGF1的无血清培养体系中继续诱导5～7d,rES细胞来源的限定性内胚层细胞分化产生约胆管样细胞。分化的细胞表达胆管细胞的特异性蛋白((CK7、CK18、CK19、CK20和OV-6)及基因(GSTPi、IB4和HNF1β)。在胆管样细胞的分化过程中检测到了Notch1和Notch2基因及下游信号分子hes1和hes5的表达。用Notch抑制剂L-685458处理分化过程中的细胞可导致Notch1和Notch2基因及下游信号分子hes1和hes5的表达下降,同时CK19阳性的胆管样细胞分化比率也从90%下降至约20%。这一... |
| 关 键 词： | 猕猴 胚胎干细胞 胆管细胞 Notch信号通路 |
Notch signaling dependent differentiation of cholangiocyte-like cells from rhesus monkey embryonic stem cells |
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| Jin Li-Fang,Ji Shao-Hui,Yang Ji-Feng,Ji Wei-Zhi. Notch signaling dependent differentiation of cholangiocyte-like cells from rhesus monkey embryonic stem cells[J]. Zoological Research, 2011, 32(4): 391-395. DOI: 10.3724/SP.J.1141.2011.04391 | |
| Authors: | Jin Li-Fang Ji Shao-Hui Yang Ji-Feng Ji Wei-Zhi |
| Affiliation: | JIN Li-Fang1,2,JI Shao-Hui2,YANG Ji-Feng2,3,JI Wei-Zhi2,*(1.College of Life Science of Shaoxing University,Shaoxing Zhejiang 312000,China,2.Kunming Institute of Zoology,the Chinese Academy of Sciences,Kunming Yunnan 650223,3.College of Life Science of Wenzhou Medical College,Shaoxing Zhejiang 325035,China) |
| Abstract: | Rhesus monkey embryonic stem(rES) cells have similar characteristics to human ES cells,and might be useful as a substitute model for preclinical research.Notch signaling is involved in the formation of bile ducts,which are composed of cholangiocytes.However,little is known about the role of Notch signaling in cholangiocytic commitment of ES cells.We analyzed the effect of Notch signaling on the induction of cholangiocyte-like cells from rES cells.About 80% of definitive endoderm(DE) cells were generated fro... |
| Keywords: | Rhesus monkey Embryonic stem cells Cholangiocytes Notch signaling |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |