| 猕猴桃L-艾杜糖脱氢酶基因的克隆及转化 |
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| 引用本文： | 梁东,邹珺,李明军,马锋旺. 猕猴桃L-艾杜糖脱氢酶基因的克隆及转化[J]. 西北植物学报, 2011, 31(11): 2147-2152 |
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| 作者姓名： | 梁东 邹珺 李明军 马锋旺 |
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| 作者单位： | 西北农林科技大学园艺学院,陕西杨陵,712100 |
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| 基金项目： | 国家自然科学基金(30871700) |
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| 摘 要： | 以猕猴桃果实为材料,利用电子克隆技术获得了L-艾杜糖脱氢酶(L-Idonate dehydrogenase,IDH)基因,然后将其转入番茄中,为了解猕猴桃IDH基因如何调控AsA的降解奠定基础。结果表明,获得的猕猴桃IDH基因cDNA全长为1 239bp,含有一个1 080bp的开放阅读框,编码359个氨基酸;成功构建了IDH基因植物表达载体pWR-IDH及工程农杆菌,以番茄叶片及茎段为受体,通过农杆菌介导法进行转化,获得了4株经PCR和RT-PCR检测呈阳性的转基因植株。
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| 关 键 词： | 猕猴桃 L-艾杜糖脱氢酶 基因克隆 遗传转化 |
Cloning and Transformation of L-Idonate Dehydrogenase Gene from Kiwifruit |
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| LIANG Dong,ZOU Jun,LI Ming-jun,MA Feng-wang. Cloning and Transformation of L-Idonate Dehydrogenase Gene from Kiwifruit[J]. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(11): 2147-2152 |
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| Authors: | LIANG Dong ZOU Jun LI Ming-jun MA Feng-wang |
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| Affiliation: | LIANG Dong,ZOU Jun,LI Ming-jun,MA Feng-wang(College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | For understanding degradation of ascorbic acid in plant,the L-Idonate dehydrogenase(IDH) gene was obtained from fruit of kiwifruit by in Silico cloning.The pWR-IDH vector was constructed and transformed into tomato.The results indicated that cDNA of IDH from kiwifruit is 1 239 bp and has an 1 080 bp open reading frame(ORF) and encodes a protein of 359 amino acid residues.The pWR-IDH vector was constructed and transformed into tomato leaves and shoot fragments by Agrobacterium tumefaciens-mediated transforma... |
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| Keywords: | kiwifruit L-Idonate dehydrogenase clonging transformation |
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