| L-异亮氨酸产生菌黄色短杆菌的选育 |
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| 引用本文： | 沈加彬,罗磊,施碧红,施巧琴,黄祥峰,吴松刚.L-异亮氨酸产生菌黄色短杆菌的选育[J].氨基酸和生物资源,2011,33(4):38-41. |
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| 作者姓名： | 沈加彬 罗磊 施碧红 施巧琴 黄祥峰 吴松刚 |
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| 作者单位： | 福建师范大学生命科学学院,福建福州,350108;福建麦丹生物集团有限公司福州研究中心,福建福州,350000 |
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| 基金项目： | 福建省自然科学基金项目(2009J01128); 福建省科技重点项目(2009N0033) |
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| 摘 要： | 以黄色短杆菌BF420为出发菌株,经过紫外线和亚硝基胍(NTG)复合诱变处理后,获得一株甲硫氨酸缺陷(Met-)及抗α-氨基丁酸(α-AB)的L-异亮氨酸产生菌BM2610,该菌株在未进行优化的发酵条件下能够积累L-异亮氨酸的量为7.12g.L-1,比出发菌株BF420提高了122.5%。
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| 关 键 词： | L-异亮氨酸 黄色短杆菌 复合诱变 育种 |
Breeding of L-Isoleucine Producing Strain of Brevibacterium Flavum |
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| SHEN Jia-bin,LUO Lei,SHI Bi-hong,SHI Qiao-qin,HUANG Xiang-feng,WU Song-gang Co.Ltd,Fuzhou,China.Breeding of L-Isoleucine Producing Strain of Brevibacterium Flavum[J].Amino Acids & Biotic Resources,2011,33(4):38-41. |
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| Authors: | SHEN Jia-bin LUO Lei SHI Bi-hong SHI Qiao-qin HUANG Xiang-feng WU Song-gang CoLtd Fuzhou China |
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| Institution: | SHEN Jia-bin1,LUO Lei1,SHI Bi-hong1,SHI Qiao-qin2,HUANG Xiang-feng2,WU Song-gang2(1.College of Life Science,Fujian Normal Univerisity,Fuzhou 350108,2.Fuzhou Research Center,Fujian Maidan Biological(Group) Co.Ltd,Fuzhou350000,China) |
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| Abstract: | Brevibacterium flavum BF420 was used as a starting strain and treated with ultraviolet(UV) and nitrosoguanidine(NTG),a mutant strain named as B.flavum BM2610,which was a methionine auxotroph and DL-α-aminobutyric acid resistance bacterium,was screened.The L-Isoleucine production of the mutant BM2610 was reached to 7.12 g·L-1 under the initial fermentation conditions,which was increased 122.5% compared to its parental strain BF420. |
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| Keywords: | L-isoleucine Brevibacterium flavum compound mutagenesis breeding |
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