| HSV-1 HF株扩增子载体的构建及其在不同HSV血清型间的通用性研究 |
| |
| 引用本文： | 宋波,刘新静,韩志强,赵璐,王青志,卢甲盟,许予明. HSV-1 HF株扩增子载体的构建及其在不同HSV血清型间的通用性研究[J]. 病毒学报, 2011, 27(5): 409-415 |
| |
| 作者姓名： | 宋波 刘新静 韩志强 赵璐 王青志 卢甲盟 许予明 |
| |
| 作者单位： | 郑州大学第一附属医院神经内科,郑州,450052;郑州大学第一附属医院神经内科,郑州,450052;郑州大学第一附属医院神经内科,郑州,450052;郑州大学第一附属医院神经内科,郑州,450052;郑州大学第一附属医院神经内科,郑州,450052;郑州大学第一附属医院神经内科,郑州,450052;郑州大学第一附属医院神经内科,郑州,450052 |
| |
| 基金项目： | 河南省卫生厅医学科技攻关项目(200801003),河南省卫生厅医学科技攻关项目(200702005); 河南省教育厅河南省基础与前沿技术研究计划项目(082300450270),河南省教育厅自然科学研究计划项目(2009A320045) |
| |
| 摘 要： | 旨在构建HSV-1HF株的扩增子载体,研究其在不同血清型HSV辅助下的包装通用性。经酶切HF株的BAC-HSV-1,获得oriS和pac元件并测序。以pSilencer2.0-U6为骨架,以DsRed为报告基因构建HSV-1HF株的扩增子载体,利用脂质体2000转染扩增子载体至Vero细胞,分别应用HSV-1HF株和HSV-2HG52辅助HSV-1扩增子载体进行包装,待产生细胞病变效应后取上清,再次感染Vero细胞,观察Vero细胞内红色荧光蛋白表达情况。本研究首次构建了HSV-1HF株的扩增子载体,鉴定了HSV-1HF株oriS和pac元件,HSV-1HF株扩增子载体可以被HSV-1HF株和HSV-2HG52株包装并扩增。
|
| 关 键 词： | 单纯疱疹病毒Ⅰ型 HSV-1 HF株 HSV-2 HG52株 复制起始点 包装信号 扩增子载体 |
Construction of the HSV-1 strain HF amplicon and study on its unversal function between different HSV serotypes |
| |
| Song Bo,Liu Xin-Jing,Han Zhi-Qiang,Zhao Lu,Wang Qing-Zhi,Lu Jia-Meng,Xu Yu-Ming. Construction of the HSV-1 strain HF amplicon and study on its unversal function between different HSV serotypes[J]. Chinese journal of virology, 2011, 27(5): 409-415 |
| |
| Authors: | Song Bo Liu Xin-Jing Han Zhi-Qiang Zhao Lu Wang Qing-Zhi Lu Jia-Meng Xu Yu-Ming |
| |
| Affiliation: | SONG Bo,LIU Xin-jing,HAN Zhi-qiang,ZHAO Lu,WANG Qing-zhi,LU Jia-meng,XU Yu-ming (The First Affiliated Hospital of Zhengzhou University Neurology,Zhengzhou,450052,China) |
| |
| Abstract: | The study aimed to construct the amplicon vector of HSV-1 strain HF and explore its universal package function between different serotypes of HSV.OriS and pac elements were obtained by enzyme digestion from the Plasmid BAC-HSV-1 strain HF and sequenced.With red fluorescence(DsRed) as a reporter gene,the amplicon vector of HSV-1 strain HF was constructed based on pSilencer2.0-U6.The amplicon vector was transfected into Vero cells by lipofectamine 2000,then packaged by HSV-1 strain HF and HSV-2 strain HG52 as... |
| |
| Keywords: | HSV-1 HSV-1 strain HF HSV-2 strain HG52 origin of replication packaging signal amplicon vector |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ |
|
