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| 墨兰多舌奇花MADS特异表达基因筛选及分析 | |
| 引用本文： | 朱永平,杨德,杨晓虹,关文灵,吴红芝,和凤美. 墨兰多舌奇花MADS特异表达基因筛选及分析[J]. 西北植物学报, 2011, 31(11) |
| 作者姓名： | 朱永平 杨德 杨晓虹 关文灵 吴红芝 和凤美 |
| 作者单位： | 1. 云南农业大学农学与生物技术学院,昆明,650201 2. 云南农业大学园林园艺学院,昆明,650201 3. 昆明学院生命科学与技术系,昆明,650031 |
| 基金项目： | 国家自然科学基金(30860178) |
| 摘 要： | 以墨兰‘南菊’为代表,用抑制消减杂交和基因芯片相结合技术构建墨兰多舌与正格花舌间的差异表达基因文库,筛选与花形态建成相关的MADS差异表达基因并构建系统进化树。结果表明:在多舌瓣中筛选出32个上调表达MADS基因,包括TM6、AG、AP1、DEF1、AP3及PI基因。其中有10个基因与AGL17、AGL29、FUI聚为一类,12个基因与其他的MADS基因归为一类。这些MADS基因参与了多舌瓣发生,并可能扮演了重要角色。该研究可为从兰花多舌变化的角度阐明墨兰奇花形成的分子机理及培育中国兰花新奇优良品种提供思路和理论依据。 |
| 关 键 词： | 墨兰 MADS基因 基因家族 系统进化树 |
Screening Specific Expression Genes of Multiple Labellum of Peloria Flower and Analysis in Cymbidium sinense |
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| ZHU Yong-ping,YANG De,YANG Xiao-hong,GUAN Wen-ling,WU Hong-zhi,HE Feng-mei. Screening Specific Expression Genes of Multiple Labellum of Peloria Flower and Analysis in Cymbidium sinense[J]. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(11) | |
| Authors: | ZHU Yong-ping YANG De YANG Xiao-hong GUAN Wen-ling WU Hong-zhi HE Feng-mei |
| Affiliation: | ZHU Yong-ping1,YANG De2,YANG Xiao-hong3,GUAN Wen-ling2,WU Hong-zhi2,HE Feng-mei2(1 College of Agriculture and Biotechnology,Yunnan Agricultural University,Kunming 650201,China,2 College of Horticulture and Landscape,3 College of Life Science and Technology,Kunming University,Kunming 650031,China) |
| Abstract: | In order to screen floral morphogenesis related differential expression MADS genes and construction of phylogenetic tree based on their DNA sequences,Cymbidium sinense cultivar 'lanjue' were employed as materials in construction of differential express libraries between multiple labella of peloria flower and labellum of normal flower by the combination of suppression subtractive hybridization and microarray.32 MADS genes were screened in multiple labella of peloria flower,including TM6,AG,AP1,DEF1,AP3 and P... |
| Keywords: | Cymbidium sinense MADS gene gene family phylogenetic tree |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |