| 酶法拆分制备D-异亮氨酸的工艺研究 |
| |
| 引用本文： | 王玉婷,吴刘洋.酶法拆分制备D-异亮氨酸的工艺研究[J].氨基酸和生物资源,2011,33(4):36-37,51. |
| |
| 作者姓名： | 王玉婷 吴刘洋 |
| |
| 作者单位： | 江苏省徐州医药高等职业学校,江苏徐州,221116;徐州信必拓生物科技有限公司,江苏徐州,221000 |
| |
| 摘 要： | 以DL-异亮氨酸为原料经过乙酰化、氨基酰化酶拆分和水解制备D-异亮氨酸.使乙酸酐与DL-异亮氨酸在0～10℃下反应生成乙酰-DL-异亮氨酸,在氨基酰化酶的作用下,温度为37℃搅拌反应3d处理得到L-异亮氨酸和乙酰-D-异亮氨酸,乙酰-D-异亮氨酸经过盐酸水解得到D-异亮氨酸,收率为97.4％,光学纯度达到98％以上.另...
|
| 关 键 词： | DL-异亮氨酸 氨基酰化酶 拆分 D-异亮氨酸 |
Study on Preparation of D-Isoleucine by Enzyme Resoluion |
| |
| WANG Yu-ting,WU Liu-yang.Study on Preparation of D-Isoleucine by Enzyme Resoluion[J].Amino Acids & Biotic Resources,2011,33(4):36-37,51. |
| |
| Authors: | WANG Yu-ting WU Liu-yang |
| |
| Institution: | WANG Yu-ting1,WU Liu-yang(1.Jiangsu Provincial Xuzhou Pharmaceutial Vacational College,Xuzhou221116,2.Xuzhou Xbetop Biotechnology Co.Ltd,Xuzhou 221000,China) |
| |
| Abstract: | Using DL-isoleucine as raw material,through acylation enzyme resolution and hydrolysis,D-isoleucine with good yield and high optical purity was obtained.DL-isoleucine and acetic anhydride were reacted in the presence of NaOH under 0-10℃ and formed N-Acetyl-DL-isoleucine.Then reacting with aminoacylase under 37℃ for three days,L-isoleucine and N-Acetyl-D-isoleucine were obtained.Hydrolysis of N-Acetyl-D-isoleucine with HCl gave D-Isoleucine with 97.4% yield and optical purity above 98%. |
| |
| Keywords: | DL-isoleucine D-isoleucine aminoacylases resolution |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
