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| 核黄素和接种番茄黄化曲叶病毒对番茄叶片防御酶活性的影响 | |
| 引用本文： | 黑银秀,朱为民,郭世荣,许爽,张辉,田守波. 核黄素和接种番茄黄化曲叶病毒对番茄叶片防御酶活性的影响[J]. 西北植物学报, 2011, 31(11): 2252-2258 |
| 作者姓名： | 黑银秀 朱为民 郭世荣 许爽 张辉 田守波 |
| 作者单位： | 1. 上海市农业科学院设施园艺研究所,上海市设施园艺技术重点实验室,上海201403;南京农业大学园艺学院,农业部南方蔬菜遗传改良重点开放实验室,南京210095 2. 上海市农业科学院设施园艺研究所,上海市设施园艺技术重点实验室,上海201403 3. 南京农业大学园艺学院,农业部南方蔬菜遗传改良重点开放实验室,南京210095 |
| 基金项目： | 上海市自然科学基金(10ZR1426900); 农业部行业计划公益性行业(农业)科研专项(201003065); 上海市科委重点基础项目(08JC1418000) |
| 摘 要： | 以番茄品种‘1479’为材料,研究了喷施核黄素(Riboflavin)和接种番茄黄化曲叶病毒(TYLCV)对幼苗叶片中过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)3种防御酶活性的影响。结果显示:(1)核黄素能显著降低番茄植株的番茄黄化曲叶病毒病的病情指数,并以2 mmol/L时诱导效果最佳,诱抗效果最高可达41.91%。(2)2mmol/L核黄素处理后96h内,番茄叶片的POD、PPO和PAL活性显著高于对照。(3)接种TYLCV后,核黄素处理和接种TYLCV处理均可诱导番茄叶片中防御酶活性显著增强。研究表明,核黄素处理可诱导POD、PPO和PAL活性的系统增强与番茄对TYLCV的诱导抗性密切相关。 |
| 关 键 词： | 番茄 核黄素 TYLCV 诱导抗性 防御酶 |
Activities of Defense Enzymes in Tomato Leaves with Riboflavin and TYLCV Inoculation |
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| HEI Yin-xiu,ZHU Wei-min,GUO Shi-rong,XU Shuang,ZHANG Hui,TIAN Shou-bo. Activities of Defense Enzymes in Tomato Leaves with Riboflavin and TYLCV Inoculation[J]. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(11): 2252-2258 | |
| Authors: | HEI Yin-xiu ZHU Wei-min GUO Shi-rong XU Shuang ZHANG Hui TIAN Shou-bo |
| Affiliation: | HEI Yin-xiu1,2,ZHU Wei-min1,GUO Shi-rong2,XU Shuang1,ZHANG Hui1,TIAN Shou-bo1(1 Protected Horticulture Research Institute Shanghai Academy of Agricultural Sciences,Shanghai Key Laboratory of Protected Horticultural Technology,Shanghai 201403,China,2 College of Horticulture,Nanjing Agricultural University,Key Laboratory of Southern Vegetable Crop Genetic Improvement,Ministry of Agriculture,Nanjing 210095,China) |
| Abstract: | Tomato cultivar '1479' were treated with riboflavin and TYLCV.The activities of peroxidase(POD),polyphenoloxidase(PPO) and phenylalanine ammonialyase(PAL) in tomato leaves were determined to study.The results showed that riboflavin can significantly lower disease index of tomato yellow leaf curl virus,and the induced resistant effect with 2 mmol/L riboflavin was the best,reached 41.91%.The activities of POD,PPO and PAL were increased significantly in tomato leaves within 96 h after spraying 2 mmol/L ribofla... |
| Keywords: | tomato riboflavin TYLCV induced resistance defense enzymes |
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