免疫荧光染色揭示草履虫接合生殖过程中静止原核的空间位置(英文)

在尾草履虫的接合生殖过程中,共有三次配前核分裂。在配前第三次分裂结束后,两个接合的细胞内均形成一个迁移原核和一个静止原核。迁移原核位于口旁锥内,而且紧贴于接合面,静止原核则位于迁移原核的外侧,两者呈左右排列,距离接近。但是,目前对导致两种原核近距离的原因尚不清楚。该文通过α-微管蛋白的单克隆抗体对受精核形成前的接合对进行了免疫荧光染色,结果发现,配前第三次分裂不同于前两次分裂,连接迁移原核和静止原核的核间连丝伸向细胞的后方,呈"U"或"V"型,结果导致两个原核左右排列,而不是前后排列,两者间的距离缩短。这个结果也阐明了造成两种原核近距离的原因。

Localization of stationary pronuclei during conjugation of Paramecium as indicated by immunofluorescence staining

After the third prezygotic division during conjugation of Paramecium caudatum, migratory and stationary pronuclei are produced. The migratory pronuclei remain in the paroral region tightly against the conjugating boundaries; while the stationary pronuclei are located beside the migratory pronuclei. To date, however, it is not clear what causes this close side-by-side localization between migratory and stationary pronuclei. In the current study, immunofluorescence staining with monoclonal antibody of anti-α tubulin indicated that 'U' or 'V' shaped spindles connected the migratory and stationary pronuclei during the third prezygotic division. This observation accounts for the close localization between these two types of pronuclei.