首页 | 本学科首页   官方微博 | 高级检索  
     


Identification of a major N-glycosylated protein of rabbit mammary gland and its appearance during development in vivo
Authors:J P Bradshaw  D A White
Affiliation:1. Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium;2. Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Ghent, Belgium;3. Mass Spectrometry Laboratory, Molecular Systems Research Unit, University of Liège, Allée du 6 Août 11, 4000, Liège, Belgium;1. NHC Key Laboratory of Glycoconjugates Research, Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Fudan University, Shanghai, 200032, China;2. Obstetrics and Gynecology Hospital, Fudan University, Shanghai, 200090, China;1. Biology Science College of Northwest Normal University, Lanzhou, 730070, China;2. New Rural Development Research Institute of Northwest Normal University, Lanzhou, 730070, China;1. Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing 100101, China;2. Key Laboratory of Intelligent Information Processing, Institute of Computing Technology, Chinese Academy of Sciences, 6 Kexueyuan South Road, Beijing 100080, China;3. University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, China;4. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Key Laboratory of Separation Science for Analytical Chemistry, Dalian 116023, China;5. Glycosciences Laboratory, Faculty of Medicine, Imperial College London, Hammersmith Campus, London W12 0NN, UK;1. Department of Cell and Molecular Pharmacology, Medical University of South Carolina, Charleston, South Carolina, USA;2. Digestive Disease Research Center, Medical University of South Carolina, Charleston, South Carolina, USA;3. Department of Obstetrics and Gynecology, Washington University in St Louis Center for Reproductive Health Sciences, St Louis, Missouri, USA;4. Department of Cancer Biology and Genetics, The Ohio State University Comprehensive Cancer Center, Columbus, Ohio, USA;5. Department of Biochemistry and Molecular Biology, Hollings Cancer Center, Medical University of South Carolina, Charleston, South Carolina, USA;6. SFR Biosit, Rennes 1 University, Rennes, France;7. Department of Biochemistry, Medical College of Wisconsin, MCW Cancer Center, Milwaukee, Wisconsin, USA
Abstract:A major N-glycosylated protein was purified from hormonally stimulated pregnant and lactating rabbit mammary tissue. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate, immunodiffusion and amino acid analysis showed the protein to be transferrin. The rate of synthesis of the protein during tissue development was studied and found to parallel the whey proteins casein and alpha-lactalbumin. The function of the protein during lactation is discussed.
Keywords:
本文献已被 ScienceDirect 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号