Mutants of phospholipase A (pPLA‐I) have a red light and auxin phenotype |
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Authors: | YUNUS EFFENDI KATRIN RADATZ CORINNA LABUSCH STEFFEN RIETZ RINUKSHI WIMALASEKERA HANNA HELIZON MATHIAS ZEIDLER GÜNTHER F E SCHERER |
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Institution: | 1. Leibniz Universit?t Hannover, Institut für Zierpflanzenbau und Geh?lzwissenschaften, Abt. Molekulare Ertragsphysiologie, , D‐30419 Hannover, Germany;2. Universit?t Kiel, Institut für Phytopathologie, , D‐24118 Kiel, Germany;3. Justus‐Liebig Universit?t Giessen, Institut für Pflanzenphysiologie, , D‐35390 Giessen, Germany |
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Abstract: | pPLA‐I is the evolutionarily oldest patatin‐related phospholipase A (pPLA) in plants, which have previously been implicated to function in auxin and defence signalling. Molecular and physiological analysis of two allelic null mutants for pPLA‐I ppla‐I‐1 in Wassilewskija (Ws) and ppla‐I‐3 in Columbia (Col) ] revealed pPLA‐I functions in auxin and light signalling. The enzyme is localized in the cytosol and to membranes. After auxin application expression of early auxin‐induced genes is significantly slower compared with wild type and both alleles show a slower gravitropic response of hypocotyls, indicating compromised auxin signalling. Additionally, phytochrome‐modulated responses like abrogation of gravitropism, enhancement of phototropism and growth in far red‐enriched light are decreased in both alleles. While early flowering, root coils and delayed phototropism are only observed in the Ws mutant devoid of phyD, the light‐related phenotypes observed in both alleles point to an involvement of pPLA‐I in phytochrome signalling. |
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Keywords: | Arabidopsis gravitropism patatin‐related phospholipase A phototropism phytochrome root coiling shade avoidance |
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