Expression and properties of the mitochondrial and cytosolic forms of fumarase in germinating maize seeds

Fumarase (EC 4.2.1.2) catalyzes reversible interconversion of malate and fumarate. It is usually associated with the tricarboxylic acid cycle in mitochondria, although the cytosolic form has also been detected. We investigated the expression of two fumarase genes and activities of the mitochondrial and cytosolic isoforms of fumarase in maize (Zea mays) scutellum during germination. Both isoforms were purified to electrophoretic homogeneity. The cytosolic form had low optimum pH (6.5) and high affinity to malate (K_m 5 μM) when compared with the mitochondrial form (optimum pH 7.0, K_m 50 μM). The cytosolic form was strongly activated by Mg²⁺ and even more by Mn²⁺, whereas the mitochondrial form was moderately activated by Mg²⁺ and Mn²⁺ was less effective. The highest fumarase activity in scutellum and a high expression of the gene encoding the cytosolic form were observed during the maximal activity of the glyoxylate cycle. In leaves, the localization of fumarase is only mitochondrial and only one fumarase gene is expressed. It is concluded that the function of cytosolic fumarase in maize scutellum can be related to metabolism of succinate formed in the glyoxylate cycle.