Rat-liver-type phosphofructokinase mRNA. Structure, tissue distribution and regulation. |
| |
| Authors: | K Hotta H Nakajima T Yamasaki T Hamaguchi M Kuwajima T Noguchi T Tanaka N Kono S Tarui |
| |
| Institution: | Second Department of Internal Medicine, Osaka University Medical School, Japan. |
| |
| Abstract: | We have cloned a full-length cDNA for rat-liver-type phosphofructokinase. The similarities of the rat liver-type phosphofructokinase mRNA to the human and mouse counterparts were 94% and 99% in their amino acid sequences and 88% and 94% in the nucleotide sequences of their coding regions, respectively. Rat liver-type phosphofructokinase mRNA was expressed in all tissues examined, but its level was regulated tissue-specifically. The nutritional and hormonal regulations of the mRNA in the liver were examined in comparison with those of two other key glycolytic enzymes, glucokinase and L-type pyruvate kinase. The level of liver-type phosphofructokinase mRNA was essentially unchanged by starvation (72 h) or diabetes. The mRNA level also did not change significantly on refeeding starved rats on a high carbohydrate diet, or treating diabetic ones with insulin. These results suggested that rat liver-type phosphofructokinase mRNA in the liver was not under control of diet or insulin, in contrast to glucokinase and L-type pyruvate kinase. |
| |
| Keywords: | |
|
|
