| 东方百合花粉萌发培养基组分的优化 |
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| 引用本文： | 车代弟,樊金萍,王金刚.东方百合花粉萌发培养基组分的优化[J].植物研究,2003,23(2):178-181. |
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| 作者姓名： | 车代弟 樊金萍 王金刚 |
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| 作者单位： | 东北农业大学, 哈尔滨 150030 |
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| 摘 要： | 以东方百合6个品种的花粉作实验材料,选用22个培养基配方,在研究硼酸和蔗糖浓度对花粉萌发和花粉管伸长影响的基础上,对东方百合花粉培养基配方进行了优化。研究结果表明东方百合离体花粉萌发培养基的最佳组成是:蔗糖13%,硼酸143mg·kg-1,琼脂1%。
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| 关 键 词： | 东方百合 花粉 培养基 |
| 收稿时间： | 2002-10-03 |
| 修稿时间： | 2002年10月3日 |
STUDY ON OPTIMIZATION OF THE CULTURE MEDIUM FOR ORIENTAL HYBRIDS POLLEN GERMINATION |
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| CHE Dai - Di FAN Jin - Ping WANG Jin - Gang.STUDY ON OPTIMIZATION OF THE CULTURE MEDIUM FOR ORIENTAL HYBRIDS POLLEN GERMINATION[J].Bulletin of Botanical Research,2003,23(2):178-181. |
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| Authors: | CHE Dai - Di FAN Jin - Ping WANG Jin - Gang |
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| Institution: | Northeast Agriculture University, Harbin 150030 |
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| Abstract: | Having adopted the six species pollen of oriental hybrids and twenty-two formulation of culture medium. We studied the effect of sucrose and boric acid concentration on the pollen germination and pollen tube elongation, and further optimized the isolated culture medium for oriental hybrids pollen germination. The result indicated that the optimum composition of isolated culture medium for oriental hybrids pollen germination was 13% sucrose+143 mg·kg-1 boric acid +1.0% agar. |
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| Keywords: | oriental hybrids pollen culture medium |
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