Effects of glucose, growth temperature, and pH on listeriolysin O production in Listeria monocytogenes. |
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Authors: | A R Datta and M H Kothary |
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Abstract: | Expression of listeriolysin O of Listeria monocytogenes as a function of different growth conditions was studied by performing a direct hemolysin assay, immunoblotting experiments, and an enzyme-linked immunosorbent assay. Expression of listeriolysin O was reduced at a lower growth temperatures (26 degrees C) and at higher glucose concentrations (> or = 0.3%) in the growth media. The effect of glucose appeared to be due to a change in the pH of the growth media. |
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