Production,isolation and partial purification of xylanases from anAspergillus sp. |
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Authors: | P. Khanna S. Sivakami Sundari N. Jothi Kumar |
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Affiliation: | (1) National Environmental Engineering Research Institute, 440 020 Nehru Marg, Nagpur, India;(2) National Environmental Engineering Research Institute, Madras Zonal Laboratory, CSIR Madras Complex, 600 113 Taramani, India |
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Abstract: | AnAspergillus sp., isolated from a rubbish dump, produced 10.6 IU ml-1 xylanase activity. Two xylanases were recognized and each was purified to homogeneity by two-stage chromatography on DEAE-and CM-Sepharose. Xylanase I had a pI of 7.2 and anMr of 26 kDa whereas xylanase II had a pI of 4.7 and anMr of 21 kDa. At 50°C, xylanase I was stable for 2.5 h but xylanase II was only stable for 1 h.P. Khanna is with the National Environmental Engineering Research Institute, Nehru Marg, Nagpur 440 020, India. S. Sivakami Sundari and N. Jothi Kumar are with the National Environmental Engineering Research Institute, Madras Zonal Laboratory, CSIR Madras Complex, Taramani 600 113, India. |
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Keywords: | Aspergillus sp. PK-7 isolation purification xylanase |
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