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Improved resolution in three-dimensional constant-time triple resonance NMR spectroscopy of proteins |
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| Authors: | Arthur G. Palmer III Wayne J. Fairbrother John Cavanagh Peter E. Wright Mark Rance |
| Affiliation: | (1) Department of Molecular Biology MB-2, The Scripps Research Institute, North Torrey Pines Road, 10666 La Jolla, CA 92037, USA |
| Abstract: | Summary Two new protocols for the three-dimensional, triple resonance, constant-time HCA(CO)N NMR experiment are presented that significantly increase the experimental resolution attainable in the C frequency dimension. Experimental verification of the new experiments is provided by spectra of the IIA domain of glucose permease fromBacillus subtilis. |
| Keywords: | 3D NMR Triple resonance Proteins Constant-time Isotopic labelling Glucose permease |
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