Rabbit T cells with and without Fc receptors |
| |
Authors: | TNS Udupa J-M Cavaillon B Cinader C-T Chou S Dubiski |
| |
Institution: | Departments of Medical Genetics, Medical Biophysics and Clinical Biochemistry and the Institute of Immunology, University of Toronto, Toronto, Ontario M5S 1A8, Canada |
| |
Abstract: | Earlier, indirect evidence for rabbit subpopulations differing in Fc receptors and in response to mitogen has been directly tested. T cells were purified from spleen suspension by removal of adherent cells, followed by removal of Ig-bearing cells on petri dishes coated with antibody, directed against the light chain allotype of Ig receptors. The purified cells were further fractionated by formation of EA rosettes and separation on Ficoll-Hypaque. T cells which lacked Fc receptors had a larger response when stimulated with Con A or PHA than did T cells which possessed Fc receptors. Both subpopulations responded more when irradiated nonadherent B cells were added to the mixture, but the extent of help was the same for both cell populations. T cells which contained both Fc receptor-bearing cells and cells which lacked the receptor had a response which was intermediate between that of the two separated subpopulations. |
| |
Keywords: | Send reprint requests to B Cinader Institute of Immunology Medical Sciences Building University of Toronto Toronto Ontario M5S 1A8 Canada |
本文献已被 ScienceDirect 等数据库收录! |