Transferrin binding by human lymphoblastoid cell lines and other transformed cells |
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Authors: | Gillian MP Galbraith Robert M Galbraith WPage Faulk |
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Institution: | Department of Basic and Clinical Immunology and Microbiology, Medical University of South Carolina, Charleston, South Carolina 29403 USA |
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Abstract: | Viable cells of 18 human cell lines, including 15 transformed cell lines of malignant and lymphoblastoid origin, were examined by an indirect immunofluorescence method for their ability to bind purified transferrin and transferrin in normal human serum. The specificity of the reaction was investigated by study of the binding reactions of several other serum proteins, including albumin, α-1-antitrypsin, and α-2-macroglobulin. Membrane binding of human transferrin was demonstrated in less than 5% of normal peripheral blood mononuclear cells or cultured diploid fibroblasts, but in more than 80% of the cells from 13 of the transformed lines, and the data obtained indicated that this binding reaction reflected the presence of specific receptors for transferrin. |
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Keywords: | Address reprints request to LCDR Thomas J Contreras MSC USN Department of Experimental Hematology Armed Forces Radiobiology Research Institute Bethesda Md 20014 |
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