BCG-induced macrophage suppression in mice: Suppression of specific and nonspecific antibody-medicated and cellular immunologic responses |
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Authors: | Denis J Schrier Elizabeth M Allen Vernon L Moore |
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Institution: | 1. Immunology Section, Research Service, Wood Veterans Administration Medical Center, Wood, Wisconsin 53193, USA;2. Departments of Pathology and Medicine, The Medical College of Wisconsin, Milwaukee, Wisconsin 53193 USA |
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Abstract: | The intravenous injection of killed BCG in an oil-in-saline emulsion (BCG-E) results in the development of intense chronic granulomatous inflammation in the lungs and spleen of C57B1/6 (B6) but not CBA mice. B6 mice injected intravenously with BCG-E exhibited marked suppression of antibody responsiveness and delayed hypersensitivity to sheep erythrocytes, as well as proliferation in response to PPD. In contrast, CBA mice similarly treated with BCG-E were not suppressed in any of these reactivities. The spleen is an important organ in this phenomenon since suppression was reversed by splenectomy and could be transferred to normal recipients with spleen cells from BCG-treated mice. Spleen cells responsible for suppression were adherent to plastic petri plates, removed with carbonyl iron, and were not eliminated with either anti-Thy-1 or anti-immunoglobulin serum + C. This study indicates that macrophages from BCG-inflamed spleen are capable of potent suppression of both antibody- and cellular-mediated immunologic reactivity. |
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Keywords: | To whom reprint requests should be addressed: Research Service/151 Veterans Administration Medical Center Wood Wisc 53193 |
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