Single bead affinity detection (SINBAD) for the analysis of protein-protein interactions |
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Authors: | Schulte Roberta Talamas Jessica Doucet Christine Hetzer Martin W |
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Institution: | Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, California, United States of America. |
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Abstract: | We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots (QDs), are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment. |
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