{alpha}-Amylase Production by Pre-mature Wheat (Triticum aestivum L.) Embryos期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

{alpha}-Amylase Production by Pre-mature Wheat (Triticum aestivum L.) Embryos

Authors:	CORNFORD, C. A. BLACK, M. DAUSSANT, J. MURDOCH, K. M.

Abstract:	Embryo/scutellar tissue of pre-mature wheat grains usually containslittle ${alpha}$ -amylase but readily produces the enzyme upon removalfrom the caryopsis. Enzyme production is accompanied by cytologicalchanges in the scutellar epithelium cells characteristic ofgerminating mature embryos, although ${alpha}$ -amylase production bypre-mature tissue is not always associated with germinativegrowth of the embryonic axis. Production of ${alpha}$ -amylase is influencedby embryo age, stimulated by GA₃ and overall is inhibited byABA. Examination by isoelectric focussing and rocket-line immuno-electrophoresisreveals the presence of both ${alpha}$ -AMYl and ${alpha}$ -AMY2 isoenzymes, thelatter being the major constituent. In the presence of ABA certain ${alpha}$ -AMY2 isoenzymes not detected previously are observed. Key words: a-Amylase, wheat, embryo

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