In vitro conservation of Bacopa monnieri (L.) using mineral oil |
| |
Authors: | Neelam Sharma Richa Satsangi Ruchira Pandey Rakesh Singh Nutan Kaushik Rishi Kumar Tyagi |
| |
Affiliation: | 1. Tissue Culture and Cryopreservation Unit, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, 110012, India 5. 1312 S Finley Road, Apt -1Q, Lombard, IL, 60148, USA 2. National Research Center on DNA Fingerprinting, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, 110012, India 3. Tata Energy Research Institute, New Delhi, 110003, India 4. Division of Germplasm Conservation, National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi, 110012, India
|
| |
Abstract: | Bacopa monnieri (L.) Wettst., a traditional Indian medicinal plant with high commercial potential, is used as a potent nervine tonic. A slow growth protocol was developed for medium-term conservation using mineral oil (MO) overlay. Nodal segments of B. monnieri (two genotypes; IC249250, IC468878) were conserved using MO for 24?months. Single node explants were implanted on MS medium supplemented with 0.2?mg?l?1 BA and were covered with MO. Subculture duration could be significantly enhanced from 6 to 24?months, on the above medium. Normal plants regenerated from conserved cultures were successfully established in soil. On the basis of 20 random amplified polymorphic DNA and 5 inter-simple sequence repeat primers analyses and bacoside A content using HPLC, no significant reproducible variation was observed between the controls and in vitro-conserved plants. The results demonstrate the feasibility of using MO for medium-term conservation of B. monnieri germplasm without any adverse genetical and biochemical effects. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|