Identification of a 24 kDa intrinsic membrane protein from mammalian peroxisomes. |
| |
| Authors: | C Reguenga M E Oliveira A M Gouveia C Eckerskorn C Sá-Miranda J E Azevedo |
| |
| Institution: | Unidade de Neurobiologia Genética do Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal. |
| |
| Abstract: | A 24 kDa protein from rat liver peroxisomal membrane was isolated and subjected to Edman degradation. Using the N-terminal sequence of this polypeptide we have identified several rat and human expressed sequence tags in the GenBank Database. The complete sequence of a human cDNA clone was determined. The open reading frame encodes an extremely basic protein 212 amino acid residues long. A high similarity between this mammalian protein and hypothetical proteins from Caenorhabditis elegans and Neurospora crassa was found. Hydropathy analysis reveals the existence of two putative membrane-spanning domains in conserved regions of the three homologous proteins. |
| |
| Keywords: | |
|
