Conformational restriction of Tyr and Phe side chains in opioid peptides: Information about preferred and bioactive side-chain topology |
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| Authors: | Dirk Tourwé Kris Verschueren Anne Frycia Peg Davis Frank Porreca Victor J Hruby Geza Toth Hendrika Jaspers Patricia Verheyden Georges Van Binst |
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| Institution: | 1. Eenheid Organische Chemie, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium;2. Department of Pharmacology, University of Arizona, Tucson, AZ, USA;3. Department of Pharmacology, University of Arizona, Tucson, AZ, USA
Department of Chemistry, University of Arizona, Tucson, AZ, USA and Biological Research Center, Szeged, Hungary |
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| Abstract: | The side chain of Tyr and Phe was fixed into the gauche (−) or gauche (+) conformation by using the Tic or Htc structures, and into the trans conformation by using an aminobenzazepine-type (Aba) structure. When incorporated into dermorphin or deltorphin II, the Tic and Htc analogues all showed a large decrease in both μ and δ affinities and activities. Fixation of Phe3 in the trans rotamer resulted in a large increase in δ affinity in the dermorphin analogue, whereas in the Aba3-Gly4] deltorphin II analogue, good δ affinity is maintained despite the removal of the Glu side chain. Whereas several authors propose a gauche (−) preferred conformation for the Phe3 side chain, these results suggest a trans conformation at the δ receptor. The use of these conformationally constrained residues for evaluating the preferred solution conformation in the flexible N-terminal tripeptide Tyr-D-Ala-Phe is illustrated. The 1H-nmr parameters—chemical shift, temperature dependence, and nuclear Overhauser effects to the D-Ala2 methyl protons in the different analogues—provide direct evidence to confirm the proposed sandwich conformation in the native peptides. © 1996 John Wiley & Sons, Inc. |
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