Protein storage in large peripheral vesicles in Phytophthora zoospores and its breakdown after cyst germination |
| |
| Institution: | 1. CAS Key Laboratory of Carbon Materials, Institute of Coal Chemistry, Chinese Academy of Sciences, Taiyuan 030001, PR China;2. Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, 19A Yuquan Road, Beijing 100049, PR China;3. College of Environmental Science and Engineering, Donghua University, Shanghai 201620, PR China;1. Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996-4561, USA;2. BioEnergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6037, USA;1. College of Chemistry, Chemical Engineering and Environment, Minnan Normal University, Zhangzhou, 363000, China;2. College of Natural Resources and Environment, Northwest A&F University, Yangling, 712100, China;3. State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Institute of Soil and Water Conservation CAS and MWR, Yangling, 712100, China;4. School of Environment and Chemical Engineering, Foshan University, Foshan, Guangdong, China;5. Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences, Guangzhou, 510000, China;6. CAS Center for Excellence in Quaternary Science and Global Change, Xi''an, 710061, China;1. Guangdong Provincial Key Laboratory for Plant Epigenetics, Shenzhen Key Laboratory of Marine Bioresource & Eco-environmental Science, Longhua Institute of Innovative Biotechnology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, Guangdong, PR China;2. Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China;3. Shaanxi Academy of Traditional Chinese Medicine, Xi’an, Shaanxi 710003, China |
| |
| Abstract: | The fate of large vesicles that line the periphery of Phytophthora cinnamomi zoospores was monitored by immunogold labeling during encystment and germination. Labeling was carried out using a monoclonal antibody, Lpv-1, directed against glycoprotein components of these vesicles. The results show that the vesicles are retained inside the zoospores during encystment, but their contents are degraded after germination. During initial stages of degradation, the large peripheral vesicles dilate to form small vacuoles containing fine electron-dense fibrillar material which is immunoreactive. Eventually, these small vacuoles fuse together to form large electron-lucent vacuoles which contain very little immunoreactive material. Immunoblot analysis of germinating cysts with Lpv-1 shows that the level of glycoprotein components in the vesicles declines dramatically following germination. It is proposed on the basis of this evidence that the large peripheral vesicles contain storage protein which acts as an endogenous supply of nitrogen reserves for the growing germ tube. Lpv-1 also labels peripheral vesicles in two other species of Phytophthora, P. parasitica var. nicotiana and P. nicotiana var. nicotiana. As in P. cinnamomi, these vesicles do not undergo exocytosis during encystment. The large peripheral vesicles thus appear to be analogous to protein bodies found in seeds of higher plants, and they may be a common feature of Phytophthora zoospores. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
