Species-specific banding patterns of restriction endonuclease-digested mitochondrial DNA from the genusPythium |
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| Affiliation: | 2. Eijkman Institute for Molecular Biology, Jl. Diponegoro 69, 10310 Jakarta, Indonesia;3. Storr Liver Centre, Westmead Millennium Institute, University of Sydney and Westmead Hospital, NSW 2145, Sydney, Australia;4. Department of Medicine, Surgery, and Health Sciences, University of Trieste, Strada di Fiume 447, 34149 Trieste, Italy;1. Microbiology and Molecular Genetics, Michigan State University, Michigan, USA;2. Biomedical Laboratory Diagnostics, Michigan State University, Michigan, USA;3. Faculty of Pharmacy, Federal University of Minas Gerais, Brazil;4. Biological Integrated Pest Management Research, Robert W. Holley Center for Agriculture & Health, Ithaca, NY, USA;1. London Research and Development Center, Agriculture and Agri-Food Canada, London, ON, N5V 4T3, Canada;2. Charlottetown Research and Development Centre, Agriculture and Agri-Food Canada, Charlottetown, PEI, C1A 4N6, Canada;3. Department of Chemistry, University of Western Ontario, London, ON, N6A 5B7, Canada;4. Department of Biochemistry, University of Western Ontario, London, ON, N6A 5C1, Canada;1. Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark;2. Department of Chemistry and Bioscience, Aalborg University, Esbjerg, Denmark;3. Commonwealth Scientific and Industrial Research Organization (CSIRO), Agriculture and Food, Queensland Bioscience Precinct, Brisbane, Australia;1. Laboratory of Plant Pathology and Biotechnology, Faculty of Agriculture, Kochi University, Nankoku, Kochi, Japan;2. Laboratory of Defense in Plant-Pathogen Interactions, Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya, Japan;3. Research Institute of Molecular Genetics, Kochi University, Nankoku, Kochi, Japan |
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| Abstract: | Restriction endonuclease-digested mitochondrial DNA from 29Pythium spp. showed distinctly different species-specific electrophoretic banding patterns. Numerical comparisons among species were conducted by calculating the percentage of restriction fragments having the same apparent molecular size. The greatest interspecific similarity in banding patterns (67%) was observed betweenHindIII digests ofPythium heterothallicum andP. sylvaticum. However, comparisons among other species generally revealed similarities of less than 50%, and often less than 30%. The lack of similarity of restriction banding patterns was observed even with several species that share many common morphological features:P. arrhenomanes vsP. graminicola (20%),P. myriotylum vsP. aristosporum (28%), andP. torulosum vsP. vanterpoolii (32%). In contrast to the fragment size heterogeneity among different species, isolates of the same species have highly conserved restriction patterns. Ten isolates ofP. oligandrum, collected from the United States, South Africa, and Czechoslovakia, had a minimum of 86% similarity inHindIII banding patterns. Similar results were observed with eight isolates ofP. ultimum, five ofP. acanthicum, six ofP. spinosum, five ofP. sylvaticum, and eight ofP. irregulare. However, two isolates ofP. irregulare exhibited a higher degree of heterogeneity and shared only 64 to 76% comigrating bands with the eight other isolates of this species. |
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