Continuous ethanol production by yeast immobilized on to channeled alumina beads |
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| Affiliation: | 1. Department of Chemistry and Chemical Biology, McMaster University, Hamilton, ON, Canada;2. Physical Sciences, Sunnybrook Research Institute, Toronto, ON, Canada;3. Department of Medical Imaging, University of Toronto, Toronto, ON, Canada;4. Centre for Probe Development and Commercialization, McMaster University, Hamilton, ON, Canada;5. Department of Materials Science and Engineering, University of Toronto, Toronto, ON, Canada;6. Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada |
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| Abstract: | Vertical and near-horizontal (15° angle) packed-bed columns were compared for continuous ethanol fermentation using an alcohol- and glucose-tolerant Saccharomyces cerevisiae strain immobilized on to channeled alumina beads (5·0 × 109 cells g−1 beads). Spaces between beads (1·0–6·5 mm) and angle (15°) of near-horizontal reactor columns (with six ports in each) efficiently removed CO2 and increased ethanol productivity. Malt-glucose-yeast-extract broth containing 16·7% glucose at 35°C fed at a dilution rate of 3· h−1 to thw two horizontal columns (in series) yielded maximum ethanol productivity of 40·0 g liter−1 h−1. Feedstock flow rate and other factors (temperature, pH, nutrients, and glucose levels) affected productivities. The immobilized-cell system showed operational stability for >3 months without plugging, and could be stored for at least one year with no loss of bioreactor performance. Scanning electron micrographs of the beads revealed large numbers of yeast-cells attached on to internal and external surfaces of beads. |
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