Abnormal cytoplasmic bundles of filaments in the Neurospora crassa snowflake colonial mutant contain P59Nc |
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| Institution: | 1. CIQUIBIC-CONICET, Departamento de Qui´mica Biolo´gica, Facultad de Ciencias Qui´micas, Universidad Nacional de Co´rdoba, Ciudad Universitaria, 5016- Co´rdoba, Argentina;2. Centro de Microscopi´a Electro´nica, Facultad de Ciencias Me´dicas, Universidad Nacional de Co´rdoba, Ciudad Universitaria, 5016- Co´rdoba, Argentina;1. Tercan Vocational High School, Erzincan Binali Yildirim University, Erzincan 24800, Turkey;2. Deparment of Chemistry, Faculty of Science, Ataturk University, Erzurum 25240, Turkey;3. Department of Chemistry, Faculty of Science and Arts, ?nönü University, Malatya 44280, Turkey;4. Bioinformatics and Genetics Department, Faculty of Engineering and Natural Sciences, Kadir Has University, Istanbul 34083, Turkey;5. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mersin University, Mersin 33169, Turkey;1. College of Resources and Environmental Sciences/Key Laboratory of Sustainable Utilization of Soil Resources in the Commodity Grain Bases in Jilin Province, Jilin Agricultural University, Changchun, 130118, China;2. Jiangsu Provincial Coordinated Research Center for Organic Solid Waste Utilization, Nanjing Agricultural University, Nanjing, 210095, China;1. State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China;2. Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, PR China;3. College of Environmental Science and Engineering, Donghua University, Shanghai 201620, PR China;1. State Environmental Protection Key Laboratory of Soil Health and Green Remediation, College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, People''s Republic of China;2. Key Laboratory of Poyang Lake Watershed Agricultural Resources and Ecology of Jiangxi Province, College of Land Resources and Environment, Jiangxi Agricultural University, Nanchang 330045, People’s Republic of China;3. Department of Plant and Soil Sciences, University of Delaware, Newark, DE, 19716, USA |
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| Abstract: | Cytoplasmic bundles of microfilaments accumulate in the Neurospora crassa morphological mutant snowflake. We have performed ultrastructural, immunoelectron, and immunofluorescence microscope studies of snowflake strains and we show here that these bundles of cytoplasmic microfilaments contain 8- to 10-nm-diameter filaments and the 59-kDa polypeptide (P59Nc) described in wild-type N. crassa strains. The immunofluorescence studies showed that almost all of the snowflake bundles are abnormal in size and morphology. Polyacrylamide gel electrophoresis of proteins from total extracts, subcellular fractions, and partially purified P59Nc from snowflake strains showed that the subcellular distribution and relative amount of P59Nc are normal in the mutants. In vitro disassembly of P59Nc bundles obtained from snowflake strains appears to occur identically to that of bundles purified from wild-type N. crassa. A polypeptide of 48 kDa enriched in a subcellular fraction of the wild-type strain was not detected in the corresponding fraction of the snowflake mutants. No significant differences in the presence or relative amounts of other polypeptides were detected between the wild-type and the snowflake strains. The results are compatible with the possibility that sn is the genetic locus either of P59Nc or of the polypeptide of 48 kDa and/or of a modifier of the P59Nc properties for in vivo supramolecular assembly in bundles of 8- to 10-nm-diameter filaments. |
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