Phosphodiesterase activity and cyclic AMP content during early germination ofMucor rouxii spores |
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| Institution: | 1. Department of Plant Physiology and Biotechnology, Nicolaus Copernicus University in Toruń, Lwowska St. 1, 87-100 Toruń, Poland;2. Department of Biology, College of Science, Mathematics, and Technology, Wenzhou-Kean University, 88 Daxue Road, Ouhai, Wenzhou 325060, Zhejiang Province, China;3. Department of Chemistry, Biology, and Biotechnology, University of Perugia, Perugia 06121, Italy;4. Wenzhou Municipal Key Lab for Applied Biomedical and Biopharmaceutical Informatics, Ouhai, Wenzhou 325060, Zhejiang Province, China;5. Zhejiang Bioinformatics International Science and Technology Cooperation Center, Ouhai, Wenzhou 325060, Zhejiang Province, China |
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| Abstract: | Incubation ofMucor rouxii spores in complex medium under aerobic conditions resulted in a very rapid transient increase in the level of soluble, low-Km cAMP phosphodiesterase. Maximum activity was reached after 2–4 min. Simultaneously, the cAMP content increased quickly, reproducing the profile exhibited by phosphodiesterase. Activation of the enzymein vitro by a cAMP-dependent phosphorylation process showed that its stimulation was minimal at those times when its activity was highest. The correlation between cellular cAMP content, phosphodiesterase activity, and sensitivity of the enzyme to activation by phosphorylation suggests that thein vivo regulation of phosphodiesterase activity by cAMP-dependent phosphorylation may be the mechanism for shutoff of the cAMP signal elicited by glucose. |
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