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Regeneration of plantlets from mature embryos of western larch
Authors:Indra S. Harry  Michael R. Thompson  Trevor A. Thorpe
Affiliation:(1) Plant Physiology Research Group, University of Calgary, T2N 1N4 Calgary, Alberta, Canada
Abstract:Larix occidentalis Nutt. can be micropropagated using whole excised mature embryos. The basal medium for induction (over 70% response) was half-strength Quoirin and LePoivre salts with Schenk and Hildebrandt organics and N6-benzyladenine singly or with kinetin at a final concentration of 10μM. Bud elongation was best on half-strength Schenk and Hildebrandt or Bornman’s mineral salts, and elongated shoots could be maintained on either half-strength Quoirin and LePoivre or Schenk and Hildebrandt formulations containing 0.05% activated charcoal, 2% sucrose, and 0.7% agar. Axillary buds developed naturally on elongating juvenile shoots, but a high sucrose treatment (6%) for 2 wk enhanced the number produced. Very good rooting (80 to 100%) was obtained by pulsing shoots for 2 to 3 h in a solution of indole butyric acid (1 mM, pH 4.5–5.0), or by planting shoots in peat-vermiculite moistened with basal medium containing 5μM α-naphthalene acetic acid, pH 5.0. Rooted shoots were hardened before transfer to the greenhouse, and initially were kept at low light and high humidity after transfer to ex vitro conditions. This research was supported initially by a National Research Council of Canada, Division of Energy Contract, and subsequently by a Natural Sciences and Engineering Research Council of Canada strategic grant to T. A. Thorpe.
Keywords:western larch   Larix occidentalis   plantlet regeneration  embryonic explants  micropropagation
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