异丙酚抑制炎性痛大鼠脊髓NOS神经元的c -fos表达

用福尔马林致痛模型、c fos基因免疫组织化学法和NADPH d组织化学技术 ,研究大鼠脊髓结构对福尔马林痛刺激的反应及异丙酚在其调节过程中的影响。结果表明 ,福尔马林痛刺激后 ,刺激侧脊髓背角出现大量Fos免疫样阳性神经元 ,其中部分为FLI/NOS双标记神经元 ;痛刺激之前或之后给予异丙酚 ,背角各层FLI神经元和FLI/NOS双标记神经元的数量均显著减少 (P <0 0 5或P <0 0 1) ;单纯腹腔注射异丙酚或生理盐水 ,脊髓未见或偶见FLI神经元。上述结果提示 :异丙酚的抗伤害作用可能与其抑制了脊髓内NOS阳性神经元的活性有关

Propofol depresses c -fos expression of NOS neurons in the spinal cord of rats with inflammatory pain

In formalin pain model, the effect of propofol on Fos expression in the spinal cord was examined by means of c -fos oncogene immunohistochemistry and NADPH-d histochemistry. Fos-like immunoreactive (FLI) neurons were mainly found in the ipsilateral dorsal horn after injection of formalin, some of which were FLI/NOS double-labeled neurons. Most of the FLI or FLI/NOS double-labeled neurons were observed in the medial part of lamina and the outer lamina . Before or after injection of formain, i.p. injection of propofol significantly decreased the number of FLI and FLI/NOS double-labeled neurons in all laminae (P<0.05 or P<0.01). By single i.p. injection of propofol or normal saline, few FLI neurons were found in the spinal cord. The results suggest that the antinociceptive function of propofol is possibly involved in the depression of the NOS neurons in the spinal cord.