补骨脂中呋喃香豆素类物质的积累规律及组织定位

应用高效液相色谱技术,分析了补骨脂(Psoralea corylifolia)不同器官、不同发育时期补骨脂素和异补骨脂素的含量变化规律。2种呋喃香豆素类物质在补骨脂的根、茎、叶、花、果实和种子中均有积累,其含量在果实中最高,根中最低,幼嫩茎叶高于成熟茎叶,成熟期果实高于幼嫩期果实。通过组织化学和荧光显微技术对呋喃香豆素类物质的定位研究,同时结合分泌腔的系数与2种呋喃香豆素的含量相关性分析,证明补骨脂中分泌腔是2种呋喃香豆素积累的主要场所。

Accumulation and Histological Localizations of Furanocoumarins in Psoralea corylifolia

The contents of two furanocoumarins in different organs and developmental stages from Psoralea corylifolia were analyzed by HPLC.The analysis indicated that psoralen and isopsoralen were accumulated in roots,stems,leaves,and seeds of P.corylifolia,with the highest content appeared in fruits and the lowest in roots.The contents of the two furanocoumarins in young shoots and leaves were higher than that in mature shoots and leaves,and the contents of the two furanocoumarins in rape fruits were higher than in young fruits.Furthermore,there was a linear relationship between furanocoumarins content and secretory cavities index on leaf in P.corylifolia.Furanocoumarins were located in secretory cavities of P.corylifolia by histochemistry and corelation analysis of the furanocoumarins content and secretory cavities index on leaf.