| Smad3基因剔除的骨髓细胞移植对小鼠的影响 |
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| 引用本文： | 陈静,沈红,赵勇. Smad3基因剔除的骨髓细胞移植对小鼠的影响[J]. 中国实验动物学报, 2010, 18(1): 9-12,I0003 |
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| 作者姓名： | 陈静 沈红 赵勇 |
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| 作者单位： | 1. 北京农学院动物科学技术系,北京,102206;中国科学院动物研究所生物膜与膜生物工程国家重点实验室,北京,100101
2. 中国科学院动物研究所生物膜与膜生物工程国家重点实验室,北京,100101 |
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| 基金项目： | 国家自然科学基金重点项目，北京市教委资助项目 |
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| 摘 要： | 目的通过小鼠骨髓细胞剔除Smad3基因,观察小鼠病理变化以及免疫T细胞状态。方法将Smad3基因剔除Smad3-/-)的小鼠骨髓细胞和野生型(Smad3+/+)小鼠骨髓细胞分别移植给60Co射线照射GFP小鼠。观察骨髓移植后GFP小鼠体征变化,第6周处死小鼠,取肠道固定,HE染色观察其病理变化,流式细胞技术检测淋巴结中T细胞变化。结果移植Smad3-/-骨髓细胞的GFP小鼠逐渐消瘦,大肠出现炎症;淋巴结中活化型的CD4+CD62LloT细胞增多。结论骨髓细胞TGF-β信号受阻,可导致小鼠患炎症疾病,引起免疫T细胞活化。
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| 关 键 词： | Smad3基因剔除^- 骨髓细胞 T细胞活化 移植 |
Impact of Smad3 Gene Knockout Bone Marrow Transplantation on Mice |
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| CHEN Jing,SHEN Hong,ZHAO Yong. Impact of Smad3 Gene Knockout Bone Marrow Transplantation on Mice[J]. Acta Laboratorium Animalis Scientia Sinica, 2010, 18(1): 9-12,I0003 |
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| Authors: | CHEN Jing SHEN Hong ZHAO Yong |
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| Affiliation: | CHEN Jing~(1,2),SHEN Hong~(1,ZHAO Yong~2(1.Department of Animal Science , Technology,Beijing University of Agriculture,Beijing 102206,China,2.State key Laboratory of Biomembrane , Membrane Biotechnology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101) |
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| Abstract: | Objective To investigate the changes of T cells and disease development in the mice with Smad3 deficiency in the bone marrow cells.Methods Bone marrow cells obtained from Smad3 null(Smad3~(-/-)) mice and wild type(Smad3~(+/+)) mice were injected to(()Co~(60))-irradiated GFP mice,respectively.The general states of the bone marrow recipients were observed.The mice were sacrificed at the sixth week and the histopathological changes in the intestines were examined.The changes of T cells from lymph nodes were de... |
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| Keywords: | Smad3~(-/-) bone marrow Activated T cells Transplantation bone marrow Mouse |
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